MACSiMAG Separator from Miltenyi Biotec

(1) Transplanted human iPSC-derived vascular endothelial cells promote functional recovery by recruitment of regulatory T cells to ischemic white matter in the brainJournal of neuroinflammationJanuary 17, 2023Xu B, Shimauchi-Ohtaki H, Yoshimoto Y, Sadakata T, Ishizaki Y.Treg expansion, the MACSiBead™ Particles were removed by MACSiMAG Separator (Miltenyi Biotec, Cat# 130–092-168). The expanded Tregs were detected by flow cytometry using a human Treg Detection Kit (Miltenyi Biotec,

(2) Multiplexed screens identify RAS paralogues HRAS and NRAS as suppressors of KRAS-driven lung cancer growthNature Cell BiologyJanuary 12, 2023Rui Tang, Emily G. Shuldiner, Marcus Kelly, Christopher W. Murray, Jess D. Hebert, Laura Andrejka, Min K. Tsai, Nicholas W. Hughes, Mitchell I. Parker, Hongchen Cai, et al.4°C for 12 hrs. The immunoprecipitates were collected using a MACSiMAG Separator (Miltenyi Biotec, 130–092-168), washed for three times with IP lysis buffer and three times with TBST. The immunoprecipitates were

(3) Integrated multi-omics reveals the activated retinal microglia with intracellular metabolic reprogramming contributes to inflammation in STZ-induced early diabetic retinopathyFrontiers in ImmunologySeptember 2, 2022Kangjia Lv, Hui Ying, Guangyi Hu, Jing Hu, Qizhi Jian, Fang ZhangFBS-containing AMES, the CD73+ cells were removed by MACS separator (Miltenyi Biotec, Germany, cat. no. 130-092-168). The cell number and cell viability were determined by Calcein Calcein AM and Propidium iodide (AM-PI)

(4) Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinomaNature communicationsJune 17, 2021Ho DW, Tsui YM, Chan LK, Sze KM, Zhang X, Cheu JW, Chiu YT, Lee JM, Chan AC, Cheung ET, et al.anti-CD3/CD28 beads (11161D, Gibco, Thermo Fisher Scientific, Waltham, USA) were removed by MACSiMAG Separator (130-092-168, Miltenyi Biotec, Germany) according to the manufacturer’s instruction before being washed once in PBS

(5) Simplified Epigenome Profiling Using Antibody-tethered TagmentationBIO-PROTOCOLJune 5, 2021Steven Henikoff, Jorja G. Henikoff, Kami Ahmad200 µl, 20 µl, and 10 µl) Strong magnet stand (e.g., Miltenyi Macsimag separator, catalog number: 130-092-168) Vortex mixer (e.g., VWR Vortex Genie) Minicentrifuge (e.g., VWR Model V) Tube rotator (e.g., Barnstead/Thermolyne

(6) Efficient low-cost chromatin profiling with CUT&TagNature ProtocolsOctober 1, 2020Hatice S. Kaya-Okur, Derek H. Janssens, Jorja G. Henikoff, Kami Ahmad, Steven HenikoffCentrifuge Eppendorf 5415R, refrigerated fixed angle rotor Macsimag magnetic separator (Miltenyi, cat. no. 130–092-168), which allows clean withdrawal of the liquid from the bottom of 0.5, 1.5 and 2 mL microfuge tubes.

(7) Exploiting macrophage autophagy-lysosomal biogenesis as a therapy for atherosclerosisNature communicationsJune 7, 2017Sergin I, Evans TD, Zhang X, Bhattacharya S, Stokes CJ, Song E, Ali S, Dehestani B, Holloway KB, Micevych PS, et al.separation column (Miltenyi Biotec 130-041-301) located in a MACS magnetic separator (Miltenyi Biotec 130-092-168). The column was washed three times with MACS buffer and, upon removal of MS column from the magnetic

(8) IκB Kinase β Is Required for Activation of NF-κB and AP-1 in CD3/CD28-Stimulated Primary CD4⁺ T CellsThe Journal of ImmunologyMarch 15, 2012Elisa Lupino, Cristina Ramondetti, Marco PiccininiIn restimulation experiments, immobilized anti-CD3/CD28 Abs were removed using MacSiMag Separator (130-092-168; Miltenyi). T cells were then washed with fresh culture medium, cultured for an additional 16 h, and

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MACSiMAG Separator

MACSiMAG Separator from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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MACSiMAG Separator from Miltenyi Biotec

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